pEASY CloNiNg VECtor

pEASY Cloning Vector
The “pEASY ” cloning vectors provide a
highly efficient, 5 minutes, one-step cloning
strategy for PCR product without the need of elimination of PCR buffers. Some of convenient features
of the vectors are as follows.
Fast
Instead of traditional method----Ligation can be accomplished without T4 DNA ligase in as little as 5
minutes hands-on time.
Easy
One microlitre PCR product and one microlitre vector are mixed together.
Mixture is incubated for 5 minutes at room temperature.
Efficient
More than 95% clones contain correct insert.
Double Selection Markers
Kanamycin and ampicillin resistance genes are used for your choice of selection in E.coli.
Easy blue/white selection
Cloning kits are supplied with free Trans1-T1 Phage Chemically Comptent Cell.
Publications with pEASY vector
Yanpeng Wang,
hexaploid brea
et al. (2014) Sim
ultaneous editin
Biotechnology,
d wheat confer
s heritable resis
[IF=39.08].
Feng Zhou et al
naling. Nature,
CRISPR-Cas syste
m. Nature Biotec
Cell Stem Cell,
ement of Oct4
rin, a canonica
antagonist, cont
[IF=10.53].
in reprogramm
ing.
F-β
s. PloS Biology,
cing reveals mut
ations in TRPV3
Journal of Hum
013) Efficient ge
als
ation in Xenopu
2) Exome sequen
Feng Z, et al. (2
C induction reve
agonist and TG
ctoderm specific
Lin Z, et al. (201
Cell Research,
l Wnt signaling
rols early neuroe
me. American
by Tet1 during iPS
oxymethylation
2) Maternal xNor
Olmsted syndro
ants using a
lation and hydr
Xu S, et al. (201
[IF=12.69].
ation of crop pl
.44].
le of DNA methy
[IF=23.315].
re
D53 regulates
genome modific
hnology, [IF= 32
. (2013) Replac
an important ro
t degradation of
[IF=38.597].
. (2013) Targeted
Yawei Gao et al
ry mildew. Natu
FD3-dependen
strigolactone sig
oeoalleles in
tance to powde
. (2013) D14–SC
Qiwei Shan et al
g of three hom
an Genetics, [IF
nome editing in
as a cause of
=11.202].
plants using a C
RISPR/Cas syste
m.
Applications:
>> Sequen
>> Cloning
cing;
l Subcloning; >> Genera
of Long PCR Fra
gments; C
T
lac Z
cin
Apa I
Aat II
Spe I
Sph I
EcoR I
BstZ I
Not I
Nco I
BstZ I
BstZ
Pst I I
Not
Sal II
pUC ori
na
Ka
cin
la
pEASY ®(383
Ampicillin
Pme I
AmSpe I
pic
illinI
Pst
lac
I
Ampicillin
cin
Kanamycin
my
na
Not I
lac
I
Not I
Spe I
Pst I
Pme I
pUC ori
pUC ori
pUC ori
f1 ori
f1 ori
f1 ori
my
A
fl ori
Spe I
EcoR I
Not I
BstZ I
Pst I
Sal I
Nde I
Sac I
BstX I
Nsi I
pU
Co
S V4
ri
Neor
Neor
Ampicillin
Neor
lac
I
Am
pic
illin
Am
pic
illin
Ampicillin
Ampicillin
lac
I
na
Ka
Am
pic
illin
na
Ka
lac
I
my
cin
pUC ori
my
na
Ka
Am
pic
illin
lac I
fl ori
lac I
Spe I
EcoR I
Not I
BstZ I
Pst I
Sal I
Nde I
Sac I
BstX I
Nsi I
Kanamycin
Spe I
EcoR I
Not I
BstZ I
Apa
PstI I
Aat
SalII I
Sph
I I
Nde
BstZ
SacI I
Nco
I I
BstX
BstZ
Nsi II
Not I
Sac II
EcoR I
Apa I
Aat II
Sph I
BstZ I
Nco I
BstZ I
Not I
Sac II
EcoR I
Apa I
Aat II
Sph I
BstZ I
Nco I
BstZ I
Not I
Sac II
EcoR I
pUC ori
pUC ori
cin
my
na
cin
Ka
my
na
cin
my
na
Ka
N eo
cin
Neor
Ka
2
or
i 2
Spe I
EcoR I
Not I
BstZ I
Pst I
Sal I
Nde I
Sac I
BstX I
Nsi I
0P
A
pU
Co
pU ri
Co
2
or ri
i
You can choose the “Blunt vectors” for a blunt-end PCR products ligation.
T
myc epitope stop
Tk p
A
Am
pic
illin
Neor
Neor
fl ori
A
0P
A
S V4
Am 0 P
SV4picililnin
icill
A mp
SP6 M13 R
T
CMV
pEASY ®-M2
(5425 bp)
pU
Co
ri
S V4
Neor
Apa I
Aat II
Sph I
BstZ I
Nco I
BstZ I
Not I
Sac II
EcoR I
in
icill
A mp
in
icill
A mp
2
Ampicillin
or
i
pEASY ®-M2
pEASY ®-Blunt3
®-M2
pEASY
(5425
bp)
（3040
bp）
®
pEASYbp)
-E2
(5425
(5393 bp)
R3
R3
pB
2
R3
2
pB
Kanamycin
pUC ori
lac I
pEASY ®-E2
pEAS
Y ®-E2
(5393
bp)
pEASY ®-Blunt Zero (5393 bp)
(3954 bp)
f1 ori
lac I
M13 F T7
T
CMV
myc epitope stop
6×His T7 term
T
T
T
T
CMV
myc
stop
6×His T7 term
Z
lac epitope
T
TT
T7
6×His T7 term
M13 F
lac O RBS ATG
TkTp
A
Tk p
A
Am
pic
illin
lac IAmp
icil
lin
Not I
Spe I
Pst I
Pme I
lac O RBS ATG
T7
fl ori
T
lac O RBS ATG
T7
fl ori
cin
my
n
Not I
T3
lac Z
Kanamycin
pUC ori
M13 F
M13 R
Kanamycin
pUC ori
T7
pEASY ®-Blunt Zero
®
pEASY ®-T5 Zero
pEASY
-Blunt
Zero
(3954
bp
)
(3954 bp)
(3953 bp)
Ampicillin
0P
A
0P
V4
pBRS3 ori
22
0
v4
Ps
pUC ori
T7
M13 F
lac Z
Ampicillin
Ampicillin
ri
Ampicillin
pB
Kanamycin
cin
pUC ori
Spe I
Pst I
I
Spe Pme
I
Not I
Pst I
Pme I
Spe I
Pst I
Pme I
Not I
mycin
pUC ori
EcoR V
BstX I
Not I
Xho I
Nsi I
Xba I
KaI na
Apa
pUC ori
na
Hind III
Kpn I
Sac I
BamH I
Spe I
BstX I
Ka
a
r m yc
in
cin
na
my
pUC ori
Am
pic
illin
na
Ka
fl ori
my
cin
pUC ori
Not I
T7
M13 F
S V4
ri
Co
pEASY ®-M1
(5437 bp)
ori
pUC
Kanamycin
lac Z
pEASY ®-T5 Zero
pEASY ®-T5 Zero
(3953 bp)
(3953 bp)
pUC ori
EcoR V
BstX I
Not I
Xho I
Nsi I
Xba I
Apa I
Hind III
Kpn I
Sac I
Hind IIIBamH I
Kpn I Spe I
Sac I BstX I
BamH I
Spe I
BstX I
EcoR V
BstX I
Not I
EcoR V
Xho I
BstX I Nsi I
Not I Xba I
Xho I Apa I
Nsi I
Xba I
Apa I
Ka
Ka
na
my
pUC ori
pUC ori
lac I
pUC ori
Spe I
Pst I
Pme I
Spe I
Pst I
Pme I
A
P
T
T7
T3 T V40
S
lac Z
Co
pU
22 ori
V5 epitope
T
Tk p
A
pEASY ®-M1
(5437 bp)
pEASY ®-M1 ®
pEASY -E1
(5437 bp)
(5715 bp)
40
0sv
v 4P
Ps
pUC ori
M13
pU R T3
M13 F
Co
M13 R T3
M13
ri R
pU
22 ori
pB R 3
T
CMV
Tk p
A
0
v4
Ps
T7
pB R 3
T
stop
40
M13 F
lac Z
Ampicillin
Ampicillin
Ampicillin
Ampicillin
T7
T
pEASY ®-Blunt Simple
(3830 bp)
pEASY ®-E1
(5715 bp)®
pEASY -E1
(5715 bp)
V5 epitope
T
CMV
V5 epitope stop
Tk
lac O RBS ATG 6×HisT pAT T T7 term
T7 term
T7
rop
T
Ampicillin
T
rop
i
A mp
pEASY ®-M2
(5425 bp)
rop
T
c iZ
llin
lac
pEASY ®-Blunt Simple
(3830 bp)
®
pEASY ®-Blunt pEASY -Blunt Simple
(3830 bp)
(3929 bp)
ri
f1 o
T
T3
Tk p
A
ri
f1 o
M13 R
ri
f1 o
T3
lac Z
S V4
Ampicillin
T
CMV
T7 term
T
lac
O RBS ATG 6×His
AAGGGC
T7 M13 TF
TTCCCG
M13 GCCCTT
F
T7
CGGGAA
SP6 M13 R
ri
in
icill
A mp
pEASY ®-T3
（3039 bp）
Ampicillin
T
lac O RBS ATG 6×His
la
0
v4
Ps
2
or
i
T7
M13 R
myc epitope c Zstop
ri
f1 o
R3
2
ri
f1 o
pB
M13 R
AAGGGC
TTCCCG
T7 M13 F
T7
or
i
Co
ori
pUC
T
GCCCTT
lac Z
CGGGAA
M13 F
2
2
M13 R
T7
R3
CMV
AAGGGC
TTCCCG
ri
T
myc epi
T
Ampicillin
6×His T7 term
Tk p
A
Ampicillin
Ampicillin
Co
i
2 o®r-Blun
pEAS2Y
(3954 bp
CMV
T
pU
lac Z
0 sv
v4 P
Ps
T
ri
f1 o
pEASY ®-E2
Ampicillin
Ampicillin
(5393 bp)
Ampicillin
T
pB
T7 M13 F
pEASY ®-T1 Simple
（3829 bp）
Ampicillin
GCCCTT
CGGGAA
lac Z
pEASY ®-Blunt
(3929 bp) ®
pEASY -Blunt
(3929 bp)
SV
pEASY ®-T3
（3039 bp）
pEASY ®-T3
（3039 bp）
ori
pUC
term
6×His T7
lac Z
T
ri
P
40
Ampicillin
i
A mp
ori
pUC
M13 R
SP6 M13TR
2
T
lac O RBS ATG
Ampicillin
pEASY ®-T1 Simple
（3829 bp）
pEASY ®-T1 Simple
（3829
bp）
A
Ampicillin
M13 R
lac Z
ri
f1 o
T7
lac Z
Co
T
pB R 3
pEASY ®-M2
pEASY ®-E2 (5425 bp)
(5393 bp)
pEASY ®-E2
(5393 bp) SP6 M13 R
T M13 F
T7
lac Z
ri
f1 o
22 ori
T7 M13 F
M13 R
(5437 bp)
pEASY ®-T1
pU bp）
（3928
Ampicillin
M13 R
lac Z
ri
f1 o
pB R 3
Ampicillin
AGGGC
TCCCG
T
lac Z
Ampicillin
ri
f1 o
（3928 bp）
pEASY ®-T1
（3928 bp）
T
ri
f1 o
ri
f1 o
rop
pEASYA -E1
mpicillin
(5715 bp)
pEASY ®-T1
pU
22 ori
6×His T7 term
lac O RBS ATG
T
T7
2
Special
technology to minimize background --100%
or efficiency
in
i
pEASY ®-M1
cill
pUC ori
M13 R
lac Z ®
M13
® R
M13 F
T
R3
T
T
k pA
GCCCT
CGGGA
stop
T
pEASY ®-Blunt
T7 M13 FZero M13 F T7
T
pEASY -T5 Zero
(3954 bp)
pEAS
Y ®-Blunt
Zero
Long
fragment
is
available
(0-10
kb)
;
GCCCT
AGGGC
T
lac Z
lac Z T
T7
T7
M13 R
M13(3953
F T
M13
F
bp)
GCCCT
T
M13
F
AGGGC
CGGGA
T7
TCCCG
T7 T M13
M13 R
F
CGGGA
TCCCG bp) T7 M13 F
T (3954
T
T7
Not I
(3953 bp)
T
lac Z
M13 R
T
Ka
T
T7
pB
EcoR V
BstX I
Not I
EcoR V Xho I
cin
BstX I Nsi I
Ka
Not I
Xba I
na
my
Xho I Apa I
cin
Ampicillin Nsi I
Xba I
Hind
ApaIIIIpUC ori
Kpn I
pUC ori
Sac I
BamH IKa
namycin
AmSpe I
pic
BstXillIin
M13 R
T
pEASY ®-T5 Zero
V5 epitope
0
v4
Ps
Hind III
Kpn I
Sac I
Hind III BamH I
lac Spe I
Kpn II
Sac I BstX I
BamH I
pUC ori
Spe I
BstX I
T
Am
piciRBS
llin ATG
lac O
M13 F
T7
pB R 3
lac Z
T
CMV
T7 term
Kanamycin
T
lac O RBS ATG 6×His
pUC ori
T7
M13 F
pEASY ®
cZ
a-E1
pEASYl®(5437
b
(5715 bp)
0
v4
Ps
M13 F
T7
T3
T3
lac Z
lac Z
T7
my
na
Ka
M13 R
(3953 bp)
Ampicillin
Spe I
Pst I
Pme I
Not I
M13 RT7 T3
M13 F
T
lac Z
Spe I
Pst I
Pme I
Spe I
Pst I
Pme I
Not I
T
Not I
Spe I
Pst I
Pme I
M13 F
pEASY ®-T5 Zero
Ka
pUC ori
my
na
Ka
f1 ori
cin
my
cin
pUC ori
pUC ori
cin
my
T
T7
T
M13 R Am
T3picillin
pEASY -Blunt Simple
(3830 bp)
M13 R
pEASY ®-E1
(5715 bp)
®
highly
Recommend
na
Ka
T3
M13 F
lac Z
Ampicillin
Ampicillin
T7
ATmpic
T
T7 ter
CMV
T
T
rop
pEASY ®-Blunt Simple
(3830 bp)
T
rop
ori
pUC
Ampicillin
T
ri
f1 o
pEASY ®-Blunt
(3929 bp)
pEASY ®-T3
（3039 bp）
T3
ri
f1 o
ri
f1 o
ri
f1 o
pEASY ®-Blunt
(3929 bp)
ri
f1 o
M13 R
lac Z
M13 R
SP6 M13 R
Kanamycin
T
lac Z
e
lac Z
lac Z
T
na
M13 F T7
Spe I
EcoR I
Not I
BstZ I
Pst I
Sal I
Nde I
Sac I
BstX I
Nsi I
M13 F
Apa I
Aat II
Sph I
BstZ I
Nco I
BstZ I
Not I
Sac II
EcoR I
T7
picillin
T
M13 FAAGGGC T7
T7 term
lac O
ATG 6×His
T7 RBS
M13 F
T7 Tlac O RBS ATG 6×His
TTCCCG
cin
lac Z
GGC
CCG
Am
AAGGGC
T7
GCCCTT
TTCCCG
M13
R
CGGGAA
GCCCTT
CGGGAA
T7 M13 F
M13 R
T7 M13 F
Ka
M13 R
EcoR V
BstX I
Not I
Xho I
Nsi I
Xba I
Apa I
M13 R
EcoR
Hind
III V
BstX I
Kpn I
Not I
Sac I
Xho I
BamH I
Nsi I
Spe I
Xba I
BstX I
Apa I
Hind III
Kpn I
Sac I
BamH I
Spe I
BstX I
Ka
na
my
Ampicillin
f1 ori
f1 ori
pUC ori
cin
pEASY ®-Blunt
(3929 bp)
na
my
na
Ka
pEASY ®-T3
（3039 bp）
my
pUC ori
cin
cin
my
na
Ka
GCCCTT
CGGGAA
M13 R
T7 M13 F
ori
pUC
pEASY ®-T1 Simple
（3829 bp）
Ampicillin
Ampicillin
T
lac Z
ri
f1 o
pEASY -T1 Simple
（3829 bp）
®
T
M13 F T7
M13 F
ri
f1 o
Ampicillin
lac Z
ri
f1 o
ri
f1 o
pEASY -T1
（3928 bp）
EcoR V
BstX I
Not I
Xho I
Nsi I
Apa I
Xba I
Aat II
Apa I
lac Z
M13 R
ri
f1 o
pUC ori
AGGGC GCCCT
T
AGGGC
F TCCCGM13 F T7 T7
R
CGGGA T7 M13
TM13 TCCCG
T
lac Z
®
Sph I
BstZ I
Nco I
BstZ I
Not I
Sac II
EcoR I
T
lac Z
pEASY ®-T1
（3928 bp）
pUC ori
Ampicillin
GCCCT
M13
F
CGGGA
M13 R T7
M13 F
Ka
lac Z
T7
T
Hind III
Kpn I
Sac I
BamH I
Spe I
BstX I
T
m
pUC ori ycin
T
M13 R T
EcoR V
BstX I
Not I
Xho I
Nsi I
Xba I
Apa I
M13 R
Hind III
EcoRI V
Kpn
BstXI I
Sac
Not I I
BamH
Xho II
Spe
Nsi I I
BstX
Xba I
Apa I
Hind III
Kpn I
Sac I
BamH I
Spe I
BstX I
Ka
na
my
pUC ori
pUC ori
ri
f1 o
pEASY ®-T1
（3928 bp）
0P
A
stop
0P
A
Product Name
pEASY®-T1 Cloning Kit
pEASY®-T1 Simple Cloning Kit
pEASY®-T3 Cloning Kit
pEASY®-Blunt3 Cloning Kit
pEASY®-T5 Zero Cloning Kit
pEASY®-Blunt Cloning Kit
pEASY®-Blunt Simple Cloning Kit
pEASY®-Blunt Zero Cloning Kit
Cat. No.
Specification
CT101-01
20 rxns
CT101-02
60 rxns
CT111-01
20 rxns
CT111-02
60 rxns
CT301-01
20 rxns
CT301-02
60 rxns
CB301-01
20 rxns
CB301-02
60 rxns
CT501-01
20 rxns
CT501-02
60 rxns
CB101-01
20 rxns
CB101-02
60 rxns
CB111-01
20 rxns
CB111-02
60 rxns
CB501-01
20 rxns
CB501-02
60 rxns
The cloning kit includes vector, Trasn1-T1 Phage Chemically Competent and etc.
Trans1-T1 Phage Chemically Comptent Cell grows fast for miniprep, shortens cloning time
and speeds up the progress of your experiment.
Tips
Competent cells at -70oC; Others at -20oC for 6 months.
Notes
Further details can be found in the customer contact below. For any questions, please contact with us by
WeChat or call TransGen Customer Service at 0086-10-57815027/57815087 or contact with our distributors.
About us
We provide high quality products for life science.
We provide simple and efficient methods.
We provide new concept for scientific research.
Website http://www.transgenbiotech.com
E-mail [email protected]
Customer Service 0086-10-57815027
Phone 0086-10-57815087
B to B Website http://transgen.en.alibaba.com
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