Stuchlíková Lucie1 , Podlipná Radka , Szotáková Barbora 1

P126
BIOTRANSFORMATION OF ALBENDAZOLE IN PLANTAGO LANCEOLATA
Stuchlíková Lucie1, Podlipná Radka2, Szotáková Barbora1, Skálová Lenka1
Department of Biochemical Sciences, Faculty of Pharmacy in Hradec Králové, Charles University in
Prague, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic1, Laboratory of Plant
Biotechnologies, Institute of Experimental Botany, Czech Academy of Science, Rozvojová 263, 165 02,
Praha 6, Czech Republic2
E-mail: [email protected]
Sekce: Proteomika a metabolomika
Albendazole (ABZ) with other benzimidazole drugs are anthelmintics widely used in
veterinary medicine in order to treat diseases in agriculture and also in human medicine
(Danaher et al., 2006). The usefulness of these drugs is uncontested, but at the same time
they represent a risk to the environment. ABZ administered to animals enter into environment
primarily through its excretion in faeces or urine. Following excretion, ABZ may persist in
the environment and impact non-target organisms. Phytoremediation is a highly promising
method that uses various plants and their associated microbes to degrade, accumulate, or
immobilize contaminants from soil and water. Plants are able to uptake xenobiotics, including
drugs, and detoxify them via biotransformation. However, only drug biotransformation into
non-toxic and stable metabolites and their consequent accumulation in plants represent drug
detoxification. For that reasons, knowledge of biotransformation pathways of drug in plants is
very important.
The objectives of this study were to identify the ABZ metabolites and ABZ
biotransformation pathways in meadow plants - cell suspensions (Plantaga lanceolata), which
commonly growth on pastures. Callus culture was achieved from primary callus rising on
stem segments of plant seedlings. Suspension cultures derived from this callus were grown.
The suspensions were incubated with ABZ (10 µM) for 24 hours. Before analysis,
homogenized samples were subjected to liquid–liquid extraction. The samples were analysed
using UHPLC/MS (QqQ) in positive-ion mode. The results showed that ABZ entered plant
cells and enzymatic systems of plant were able to transform ABZ via several reactions.
This project was supported by Czech Science Foundation, grant No.15-05325S
Danaher M, H. L., Crooks SRH, Cerkvenik-Flajs V, O´Keeffe M (2006). "Review of
methodology for the determination of macrocylic lactone risidues in biological matrices."
Journal of Chromatography B 844: 175-203.