Prezentace Ing. Martina Kropáčka, Ph.D. na téma

Prezentace Ing. Martina Kropáčka, Ph.D. na téma

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  Konjugace protilátky Nimotuzumab (hR3) s bifunkčním ligandem DOTA
Podmínky konjugace
  Molární poměr hR3:Ligand je 1:10
  Prostředí 0,1 M PBS
  Inkubace 2 h, LT
  Mírné třepání (450 rpm)
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  Po 1 h reakce kontrola pH
⇒ kritický parametr !!
  Stop reakce – 1,5 M TRIS/HCl, pH 8,88
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nRIU *1000
Sig A
0,0
-20,0
5,00
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40
20
0
0,00
10,00
15,00
min
hR3-konjugat
-40,0
0,00
5,00
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10,00
15,00
min
CPS
ChB
30
25
20
15
0,00
5,00
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10,00
15,00
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Kontrola procesu purifikace pomocí HPLC s UV detekcí při 280 nm
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0,00
5,00
mAU
40
20
0
0,00
10,00
15,00
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ELIMINACE
STUDIE
  Krevní clearance - odběry 10 min, 30 min, 1, 6, 24, 48, 72 a 168 hod
  Renální exkrece – odběry 4, 8, 16, 20 a 24 hod
DISTRIBUCE
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168 hod
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Maximum: 63,5% 48h
Průměr: 34%
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Nárůst na 3,61 % za 48h
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Pokles na : 0,553 %
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