(19) United States Jun. 27, 2013

US 20130164292A1
(19) United States
(12) Patent Application Publication (10) Pub. No.: US 2013/0164292 A1
Boyle et al.
(43) Pub. Date:
Jun. 27, 2013
(54)
HUMAN ANTI-OPGL NEUTRALIZING
ANTIBODIES AS SELECTIVE OPGL
PATHWAY INHIBITORS
Related US. Application Data
(62) Division of application No. 12/782,376, ?led on May
18, 2010, noW Pat. No. 8,367,063, Which is a division
of application No. 10/408,901, ?led on Apr. 7, 2003,
(71) Applicants:Amgen, Inc., Thousand Oaks, CA (US);
noW Pat. No. 7,718,776.
Medarex, Inc., Princeton, NJ (US)
(72) Inventors: William J. Boyle, Malibu, CA (US);
(60) Provisional application No. 60/370,407, ?led on Apr.
5, 2002.
Eugene Medlock, Westlake, CA (US);
Publication Classi?cation
John K. Sullivan, NeWbury Park, CA
(US); Robin L. Elliott, NeWbury Park,
CA (US); Frank Martin, NeWbury Park,
CA (US); Haichun Huang, Fremont,
CA (US)
(51)
Int. Cl.
(52)
US. Cl.
C07K 16/18
CPC
(73) Assignees: MEDAREX, INC., PRINCETON, NJ
(US); AMGEN, INC., THOUSAND
OAKS, CA (US)
(21) Appl. No.: 13/729,816
.. ...
USPC
(57)
(2006.01)
. . . . ..
C07K16/18 (2013.01)
................................... .. 424/135.1; 424/174.1
ABSTRACT
Monoclonal antibodies and hybridomas producing them that
interact With osteoprotegerin ligand (OPGL) are provided.
Methods of treating osteopenic disorders by administering a
pharmaceutically effective amount of antibodies to OPGL are
(22)
Filed:
Dec. 28, 2012
also provided. Methods of detecting the amount of OPGL in
a sample using antibodies to OPGL are further provided.
Patent Application Publication
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Jun. 27, 2013 Sheet 2 0f 26
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Patent Application Publication
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Jun. 27, 2013 Sheet 4 0f 26
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Patent Application Publication
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Patent Application Publication
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Jun. 27, 2013 Sheet 7 0f 26
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Patent Application Publication
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Jun. 27, 2013 Sheet 8 0f 26
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Patent Application Publication
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Jun. 27, 2013 Sheet 9 0f 26
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Patent Application Publication
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@NH om: owm com mwm
Jun. 27, 2013 Sheet 10 0f 26
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Patent Application Publication
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Jun. 27, 2013 Sheet 12 0f 26
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Patent Application Publication
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Jun. 27, 2013 Sheet 13 0f 26
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Patent Application Publication
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Jun. 27, 2013 Sheet 14 0f 26
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Patent Application Publication
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Jun. 27, 2013 Sheet 15 0f 26
@NH om: owm com wm
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US 2013/0164292 A1
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Patent Application Publication
Jun. 27, 2013 Sheet 17 0f 26
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Jun. 27, 2013 Sheet 18 0f 26
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Patent Application Publication
Jun. 27, 2013 Sheet 19 0f 26
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