podrobný program konference | pdf

SATURDAY, OCTOBER 3, 2015
HALL A | EVROPA 1
19.00 – 19.30
1.
Opening
Chairmen: Doležel J., Kalina T.
Opening Lecture
Robinson J.P. | West Lafayette, Indiana, USA
FOYER
19.30 - 21.30
Welcome Drink
SUNDAY, OCTOBER 4, 2015
HALL A | EVROPA 1 | PLENARY SESSIONS
8.30
Opening Ceremony
8.55 – 10.25
Future of Cell Analysis
Chairmen: Souček K., Kalina T.
2.
Spectral Cytometry – Is This the Future or Is It Now?
Robinson J.P. | West Lafayette, Indiana, USA
(35´)
3.
Single Cell Analysis on Microfluidic Devices
Dittrich P. | Zürich, CH
(35´)
4.
CompanyPresentation:PlatinumPartner-BeckmanCoulter Beckman Coulter Flow Cytometry in 2015 and Beyond
Jaen O.
(20´)
10.25 – 10.45
Coffee Break
10.45–12.15 CytometryInspiration
Chairmen: Hofmanová J., Říhová L.
5.
Flow Cytometry Detection of Microparticles in Whole
Blood: A Novel Approach
Manganaro D. | Milano, IT
(35´)
6.
Challenges of Minimal Residual Disease Testing and its Promise for Multiple Myeloma Therapeutics
Wallace P.K. | Buffalo, NY, USA
7.
Company Presentation: Gold Partner - BD Biosciences | I.T.A. - Intertact s.r.o.
New Tools in Flow Cytometry for Research and Diagnostics
(15´)
Loehr A.
8.
Company Presentation: Silver Partner – Bio-Rad
How the Biorad S3e Cell Sorter Simplifies the Science of Sorting (10´)
Astruc C. | Business Development Manager CBBU
12.15 – 13.45
Lunch / Lunch Sessions
13.45 – 15.35 Cytometry Investigation of Mice and Man
Chairmen: Průcha M., Drbal K.
9.
(35´)
Application of Flow Cytometry in the Diagnostics of Allergy
Knol E. | Utrecht, NL
(35´)
10. The Immunophenome of the Mice - Integrative Multiparametric Approach
Luche H. | Marseilles, FR
(35´)
Company Presentation: Silver Partner – Miltenyi Biotec GmbH | Biohem
(10´)
11. REAfinityTM and Vio®Dye Antibodies
Goczalik-Schuster I.
Company Presentation: Silver Partner – Merck Millipore
12. Fast flow of Recent Merck Millipore Innovations for Flow Cytometry
Kukla S.
(10´)
Company Presentation: Silver Partner – Accela
13. High-End Cytometric Solutions for Cancer Research
Pasculli R.
(10´)
Company Presentation: Partner – Life Technologies Czech Republic
14. Attune NxT: The Next Generation Acoustic Flow Cytometer
Brey A.
(8´)
15.35 – 15.55
Coffee Break
15.55 – 17.10 Back to Origins
Chairmen: Eckschlager T., Kanderová V.
15. Toll-Like Receptors Expressed on Embryonic Macrophages Couple Inflammatory Signals to Iron Metabolism During Early Ontogenesis
Balounová J., Vavrochová T., Dobešová M., Ballek O., Kolář M.,
Filipp D. | Prague, CZ
(20´)
16. The Transmembrane Protein EVI2B Regulates Hematopoietic (20´)
Stem Cell Function
Zjablovskaja P.1, Kardošová M.1, Brdička T1., Benoukraf T.2, Tenen D.2, Malissen
B.3, Balaštík M.1, Hořejší V.1, Alberich-Jorda M.1 | 1Prague, CZ, 2Singapore, SG,
3
Marseille, FR
Company Presentation: Partner – EXBIO Praha
17. EXBIO aneb výroba protilátek v Čechách
Špryngar M.
(8)
Company Presentation: Partner – Biolegend
18. Effective Magnetic Cell Separation with MojoSort™
Fröhlich A.
(8´)
Company Presentation: Partner – Olympus
19. Scan R – Microscopic System for High Content Screening
(8´)
Jendrulek T.
17.00 – 17.10 Award Ceremony – The Best Cytometric Publication 2014
SUNDAY, OCTOBER 4, 2015
HALLP|OLOMOUC1+2+3
17.10–18.10
PosterPresentationsI
Moderatedposterwalkforauthorsofodd-numberedposters
(Posters P1, P3, P5 …)
MONDAY,OCTOBER5,2015
HALLP|OLOMOUC1+2+3
17.05–18.00
PosterPresentationsII
Moderatedposterwalkforauthorsofeven-numberedposters
(Posters P2, P4, P6 …)
MONDAY,OCTOBER5,2015
HALL A | EVROPA 1 | PLENARY SESSION
8.30–10.35
StoriesToldbyCells
Chairmen: Doležel J., Vlková M.
20. Cytometry of Immunological Memory and Immunity
Radbruch A. | Berlin, DE
(35´)
21. The Private Life of Plants: New Insights Provided by DNA
Flow Cytometry
Suda J. | Prague, CZ
(35´)
22. Application of Flow Cytometry in Protein and Metabolic Engineering
(20´)
Prokop Z.1, Fedr R.1, Buryška T.1, Dvořák P.1, Chrást L.1, Fischlechner M.2, Nikel P. I.3,
Chaloupková R.1, Hollfelder F.4, de Lorenzo V.3, Souček K.1, Damborský J.1 | 1Brno,
CZ, 2Southampton, UK, 3Cantoblanco-Madrid, ES, 4Cambridge, UK
CompanyPresentation:Partner–BIO-PORTEurope
23. eBioscience PrimeFlow™ RNA Assay – Simultaneous Detection
of RNA and Proteins on Single Cell Level
Hovorka O.
CompanyPresentation:GoldPartner–Scintila
24. Spectral Analysis in Flow Cytometry
Piedavent-Salomon M. | Sony Europe
CompanyPresentation:Partner–KRD
25. Across Living Colors Fluorescent Proteins and Cytometer Calibration
Beads in Recent Science
Lorencová M.
10.35 – 10.55
(8´)
(15´)
(8´)
Coffee Break
HALL A | EVROPA 1 | PARALLEL SESSION I
10.55–13.10
CellBiologyandSignalling
Chairmen: Jaroš J., Kozubík A.
26. Variability in the Cellular Response to Activation of P53
on the Genome-Wide Scale
Andrysik Z., Galbraith M.D., Allen M.A., Azofeifa J.G., Dowell R.D.,
Espinosa J.M. | Boulder, CO, USA
(20´)
27. Glycoprotein Asporin is Upregulated By Three-Dimensional
(20´)
Growth and Promotes Invasion of Triple-Negative Breast Cancer Cells
Bouchal J., Simkova D., Kharaishvili G., Ozdian T., Soukup T., Krupka M.,
Galandakova A., Dzubak P., Mickova A., Korinkova G. | Olomouc, CZ
28. The Transcription Factor C/EBPγ is Dispensable for Hematopoiesis
Kardošová M., Zjablovskaja P., Alberich-Jorda M. | Prague, CZ
(20´)
29. Valproic ACID Increases Cd133 Positive Cells that Show Stem Cell
(20´)
Features in Neuroblastoma
Khalil M.A., Hraběta J., Groh T., Procházka P., Doktorová H., Eckschlager T.
| Prague, CZ
CompanyPresentation:GoldPartner–BioTech
30. Think Antibody, Think Specific
Think Imaging, Think Possible With Cytation
Rego A., Fukalová J.
(15´)
31. Role of IFNalpha and IL-10 Dysregulation in Central Nervous System
(20´)
in Transgenic Porcine Huntington’s Disease Model
Valekova I.1, 2, Jarkovska K.1, Kotrcova E.1, 2, Bucci J.3, Ellederova Z.1, Juhas S.1,
Motlik J.1, Gadher S.J.4, Kovarova H.1 | 1Libechov, CZ, 2Prague, CZ, 3Carlsbad, CA,
USA, 4Maryland, USA
32. New Challenges in the Treatment of Failing Heart: Focus
(20´)
on Inflammatory Responses of Innate Immune Cells
Pekarová M.1, Ambrožová G.1, Martišková H.1, Koudelka A.1, Lojek A.1, Klinke A.2,
Rudoplh T.K.2, Kadlec J.1, Trundova M.3, Kuchta R.1, Kuchtova Z.1, Kubala L. | 1Brno,
CZ, 2Cologne, DE, 3Prague, CZ
12.50 – 14.15
Lunch / Lunch Sessions
HALL A | EVROPA 1 | EDUCATIONAL SESSION I
14.15 – 17.05
Image Analysis
Chairman: Souček K., Ešner M.
33. Basics of Image Analysis
Jaroš J. | Brno, CZ
34. Image Processing - Concepts and Methods
Fedr R. | Brno, CZ
35. Optimization of Live Cell Imaging
Ešner M. | Brno, CZ
Discussion
15.55 – 16.15
Coffee Break
HALLA||EVROPA1|CSACMEMBERSONLY
18.00–19.00
PlenaryMeetingoftheCzechSocietyforAnalyticalCytology
Chairmen: Kalina T., CSAC Committee
MONDAY,OCTOBER5,2015
HALL B | EVROPA 2 | PARALLEL SESSION II
10.55 – 12.50
Botany I
Chairmen: Bureš P., Vrána J.
36. Flow Cytometric Detection of Holocentric Chromosomes in Plants
Bureš P., Veselý P., Zedek F. | Brno, CZ
(20´)
37. Development of High-Lipid Content Strains of Microalgae
Chlamydomonas Reinhardtii by Fluorescence-Activated Cell Sorting
Búzová D., Fedorko J., Červený J. | Drásov, CZ
(20´)
CompanyPresentation:GoldPartner–BioTech
38. Think Antibody, Think Specific
Think Imaging, Think Possible With Cytation
Rego A., Fukalová J.
39. Representative Amplification of DNA from Single Plant Chromosomes
Cápal P., Blavet N., Vrána J., Kubaláková M., Doležel J. | Olomouc, CZ
(15´)
(20´)
40. 25-Fold Genome Size Difference in Genlisea – What Makes These
(20´)
Genomes Different?
Tran T.D.1, Cao H.X.1, Jovtchev G.1, Neumann P.2, Novák P.2, Fojtová M.3,
Vu G.T.H.1, Macas J.2, Fajkus J.3, Schubert I.1, Fuchs J.1 | 1Gatersleben, DE,
2
České Budějovice, CZ, 3Brno, CZ
41. Residual Sexuality in Supposed Apomicts: The Case of Sorbus
Polyploids
Lepší M.1, Koutecký P.1, Bílá J.2, Lepší P.3, Vít P.4 | 1České Budějovice, CZ,
2
Praha, CZ, 3Český Krumlov, CZ, 4Průhonice, CZ
12.50 – 14.15
Lunch / Lunch Sessions
14.15 – 15.55
Botany II
Chairmen: Suda J., Šmarda P.
(20´)
42. Cell-Type-Specific Cytokinin Distribution Within the Arabidopsis
(20´)
Primary Root Apex
Antoniadi I.1,2, Plačková L.3, Simonovik B.1, Doležal K.3, Turnbull C.2, Ljung K.1,
Novák O.3 | 1Umeå, SE, 2London, UK, 3Olomouc, CZ
43. Flow Cytometry Teams Up With Mass Spectrometry to Unlock
(20´)
the Plant Nuclear Proteome
Petrovská B., Jeřábková H., Chamrád I., Vrána J., Lenobel R., Uřinovská J.,
Šebela M., Doležel J. | Olomouc, CZ
44. Bionano Genome Mapping of Flow-Sorted Chromosomes (20´)
Supports Physical Mapping and Sequence Assembly in Complex Plant Genomes
Staňková H.1, Hastie A.2, Vrána J.1, Kubaláková M.1, Visendi P.3, Hayashi S.4,
Luo M.C.5, Batley J.4, Edwards D.3, Doležel J.1, Šimková H.1 | 1Olomouc, CZ,
2
San Diego, USA, 3Brisbane, AU, 4Brisbane, AU, 5Davis, USA
45. Genomic GC Content Variation in Plants(20´)
Šmarda P., Březinová A., Bureš P., Veselý P. | Brno, CZ
46. The Role of Whole-Genome Processes in the Origin (20´)
of the Hyperdiverse Cape Flora
Urfus T.1,2, Trávníček P.2, Bílá J.1, Vítová J.2, Chumová Z.1,Hanušová K.1,
Suda J.1,2 | 1Prague, CZ, 2Průhonice, CZ
15.55 – 16.15
Coffee Break
MONDAY,OCTOBER5,2015
HALLC|MADRID1|EDUCATIONALSESSIONII|CZECHONLY
14.15–17.05
Klinickácytometrie|CZ
Předsedající: Vlková M., Kanderová V.
47. Kontrola kvality v klinické cytometrii aneb Jak se vyrovnat s ISO15189?
Vlková M.1, Kanderová V.2 | 1Brno, CZ, 2Praha, CZ
48. Jak validovat a verifikovat cytometrická vyšetření?
Vlková M.1, Kanderová V.2 | 1Brno, CZ, 2Praha, CZ
Problémy,sekterýmisevklinicepotýkáme:
49. Titrace protilátek a kitů – co si můžeme dovolit?
Vášková M. | Praha, CZ
50. Jak správně stanovit HLA-B27?
Kryštůvková O. | Praha, CZ
51. CD8 Escapees aneb jak neztratit CD8 pozitivní T-lymfocyty?
Vášková M., Kanderová V. | Praha, CZ
52. Co můžeme zjistit z vyšetření leukocytárních subpopulací v periferní krvi?
Vlková M., Nechvátalová J. | Brno, CZ
53. Částečný IgD deficit
Nechvátalová J., Chovancová Z. | Brno, CZ
15.55 – 16.15
Coffee Break
MONDAY,OCTOBER5,2015
HALLD|MADRID2|EDUCATIONALSESSIONIII
14.15–17.05
FACSSorting
Chairman: Šinkora J., Vrána J.
54. Sorting vs. Analysis – Thinking Out of the Box
(50´)
Presentation about sorting, including tips,&tricks and extra panel design for
sorting.
Schlender J. | Erembodegem, BE
55. Sorting Out Sorting Issues
What should the sorter operator know about your soting experiment.
Semerák P., Kalina T. | Prague, CZ
(20´)
56. The BD FACSAria Fusion – Thinking In The Box
Presentation focusing on Aria Fusion & Biosafety.
Schlender J. | Erembodegem, BE
(20´)
57. What Can an Application Specialist Advice a FACSAria User?
Experience form the field, configurations, Firmware tuning, standardization.
Šinkora J. | Prague, CZ
(20´)
58. What We Are Proud of and What We Have Been Coping with
(40´)
Representatives of Individual Laboratories Will Be Requested to Prepare a Short
Presentation About Their Top Applications and Successes.
Their comments on bottlenecks and troubleshooting tips will be welcome.
Sorter users
15.55 – 16.15
Coffee Break
TUESDAY, OCTOBER 6, 2015
HALL A | EVROPA 1 | PARALLEL SESSION III
8.30–10.30
CytometryinTranslationalResearch
Chairmen: Fišer K.
59. Cell of Origin Search in Bladder Carcinoma: Cell Surface Phenotype
(20´)
and Chemosensitivity Analyzed by Multivariate Cytometry
Drbal K., Müller N., Jariwala M-S., Matěj R., Stejskal J., Záleský M., Vicenová K.,
Zachoval R. | Prague, CZ
60. Expression and Relevance of Cancer Stem Cell Markers in Colorectal
Cancer
Matúšková M., Ďuriníková E., Kozovská Z., Kučerová L. | Bratislava, SK
(20´)
61. Analysis of Fluorescently Labeled Microvesicles and Exosomes Using
(20´)
Dedicated Flow Cytometry
Pospichalova V., Svoboda J., Dave Z., Kotrbová A., Kaiser K., Klemova D.,
Ilkovics L., Hampl A., Crha I., Jandakova E., Minar L., Weinberger V., Bryja V. | Brno, CZ
62. Diagnostic Features Extracted from EuroFlow PID Dataset
(20´)
for Evaluation of Primary Immunodeficiency
Stuchlý J.1, Kalina T.1, Šinkorová V.1, Mejstříková E.1, Nováková M.1, Vlková M.1, van
Zelm M.C.3, Perez-Andres M.4, Lopez E.5, Orfao A.4, van Dongen J.J.M.3, van der
Burg M.3 | 1Praha, CZ, 2Brno, CZ, 3Rotterdam, NL , 4Salamanca, ES, 5Madrid, Spain
63. Melanoma Regression in MeLiM Pigs Is Associated with Expansion
(20´)
of CD4+CD8+ T-Cells
Sinkorova Z.1,2, Kucerova P.1, Cizkova J.1, Horak V.1, Planska D.3, Krizan J.1,
Cervinkova M.1, Sinkora M.4, Sinkora J.4 | 1Liběchov, CZ, 2Hradec Králové, CZ,
3
Prague, CZ, 4Prague,CZ
64. EuroFlow Quality Assurance Program: Proposal for Structure
(20´)
and Implementation
Kalina T.1, Flores-Montero J.2, Lecrevisse Q.2, Pedreira C.3, van der Velden V.H.J.4,
Novakova M.1, Mejstrikova E.1, Hrusak O.1, Böttcher S.5, Karsch D.5, Sędek Ł.6,
Trinquand A.7, Boeckx N.8, Caetano J.9, Asnafi V.7, Lucio P.9, Lima M.10, Santos
A.H.10, Bonaccorso P.11, van der Sluijs-Gelling A.J.12, Langerak A.W.4, Martin-Ayuso
M.2, Szczepański T.6, van Dongen J.J.M.4, Orfao A.2 | 1Prague, CZ, 2Salamanca, ES,
3
Rio de Janeiro, BR, 4Rotterdam, NL, 5Kiel, DE, 6Zabrze, PL, 7Paris, FR, 8Leuven, BE,
9
Lisbon, PT, 10Porto, PT, 11Monza, IT, 12The Hague, NL
10.30 – 10.50
Coffee Break
HALL A | EVROPA 1 | EDUCATIONAL SESSION IV
10.50 – 12.20
Cell Biology
Chairman: Kalina T., Wallace P.K.
65. Introduction
Wallace P.K. | Buffalo, NY, USA
66. Combined Detection of Surface and Intracellular Antigens: Opportunities and
Obstacles
Šimečková Š. | Brno, CZ
67. Fluorescent Barcoding in Facs-Based Profiling of Cell Surface Antigen Expressions
Remšík J. | Brno, CZ
68. Phospho Flow in Immunodeficiency
Kanderova V. | Prague, CZ
69. Multicolor Phenotyping With Proliferation Dyes and Viability Dyes
Wallace P.K. | Buffalo, NY, USA
12.20 – 12.35
Closing Ceremony
Chairmen: Kalina T., Doležel J.
12.35
Lunch
TUESDAY, OCTOBER 6, 2015
HALL B | EVROPA 2 | PARALLEL SESSION IV
8.30–10.30
ClinicalCytometry
Chairperson: Vášková M.
70. Molecules Exhibiting Dipeptidyl Peptidase-LV-Like Enzymatic Activity
in Local and Systemic Pathogenetic Processes of Rheumatoid Arthritis
Sromova L., Busek P., Mareckova H, Sedova L., Sedo A. | Prague, CZ
(20´)
71. KV1.3 Lymphocyte Potassium Channel Inhibition as a Potential
(20´)
Novel Therapeutic Approach in Acute Ischemic Stroke
Bajnok A., Folyovich A., Biró E., Orbán C., Vásárhelyi B., Toldi G. | Budapest, HU
72. Flow Cytometry In Bone Marrow Involvement with Mantle Cell
(20´)
Lymphoma – Correlations with Cytomorphology, Trephine Biopsy and Genetics
Gyarfas J.1, Mlcakova A.1, Plank L.2, Szepe P.2, Balharek T.2, Marcinek J.2, Leitnerova
M.1, Copakova L.1 | 1Bratislava, SK, 2Martin, SK
73. Different Immunological Characteristics of Cord Blood Cells
(20´)
of Newborns of Healthy and Allergic Mothers
Hrdý J., Novotná O., Vlachynská K., Prokešová L. | Prague, CZ
74. B-Lymphocyte Maturation and T-Independent Response in Patients
(20´)
with Digeorge Syndrome
Klocperk A., Kayserová J., Mejstříková E., Kalina T., Šedivá, A. | Prague, CZ
75. Phenotype Profile of Waldenström Macroglobulinaemia
(20´)
Rihova L.1, Vsianska P.1, Bezdekova R.1, Suska R.1, Stejskalova M.1, Adam Z.1, Penka
M.1, Hajek R.1,2 | 1Brno, CZ, 2Ostrava, CZ
10.30 – 10.50
Coffee Break
HALLB|EVROPA2|EDUCATIONALSESSIONV|CZECHONLY
10.50–12.20
Základypoužitícytometrie|CZ
Chairman: Janda J., Šinkora J.
76. Technické základy
Svoboda J. | Praha, CZ
77. Fluorescence a kompenzace
Janda J. | Praha, CZ
78. Standardizace
Šinkora J. | Prague, CZ
79. Pravidla pro design vícebarevných panelů
Vlček R. | Prague, CZ
12.35
Lunch