09_ECFS Case Study Workshop

X
(slovami: plnoletý !!!)
Vysoké Tatry, 11.6. – 13.6. 2014
Serum No. 1
A
A
Test
Centromere (IIFT)
Result
FP1
3
b2
FP2
FP3
Conjugate
Final Result
IgG
3
0
0
Fluorescence pattern not listed in the pull-down menu
ELISA
Test
Result
Crith.
FARR-assay
IU
Result
IU
IIFT
ds-DNA
Other Method
Method
Result
Final Result
IU
2
2
Other method
Test
ELISA
ELISAc
IB
ID
CIE
ALBIA
b
+
K
$
#
"
!
#
2
RNA polymerases
b
Cardiolipin IgA
!2-Glycoprotein IgG
)
(
'
&
Cardiolipin IgM
NOR-90
R
U1-snRNP
*
SS-B/La
Th/To (RNase P)
C
Coilin p80
- SS-A/Ro 60
%
Ratio: specific
result/cut-off value
assay
F
- SS-A/Ro 52
Result
Final Result
S
S
Method
natural /
recombin
antigen
2-Glycoprotein IgM
- U1-A
- Ku 70
Phospathidylserin IgG
- U1-C
- Ku 80
Phospathidylserin IgM
Sm
Mi-2
- Sm B'B
His-tRNA synthetase (Jo1)
- SmD
Ala-tRNA synthetase (PL12)
Ribosomes P0, P1, P2
PCNA
SL/Ki
C1q
Histones
Nucleosomes
CENP A
CENP B
CENP C
Scl-70/Topo I
PM/Scl
- PM/Scl-75
- PM/Scl-100
Ileu-tRNA synthetase (OJ)
Thr-tRNA synthetase (PL7)
Gly-tRNA synthetase (EJ)
Tryp-tRNA synthetase
Asp-tRNA synthetase
Signal recognition particle
C-ANCA
Prothrombin IgG
Prothrombin IgM
Annexin IgG
Annexin IgM
Citrulline (ACPA)
RF-IgM
RF-IgG
RF-IgA
RF non-isotype
P-ANCA
RA-33
atypical ANCA
Mitochondria type M2
PR3-ANCA
Nuclear pore complex gp210
MPO-ANCA
Sp 100
GBM
Other antigens
!
!
!
!
!
!
!
!
!
!
!
!
!
5
1
/
,
9
9
0
8
5
4
9
0
/
0
8
8
0
,
7
<
1
;
8
:
1
B
3
1
@
Negative
Unknown
1
f1
g1
U
?
>
H
=
c
D
c
Nucleolar patterns
c1
Homogeneous
ogeneous nucleolar
Clumpy nucleolar
c3
Punctate nucleolar
Centriole
Spindle pole (NuMa)
Spindle fibre
Midbody (MSA-2)
CENP-F (NSp-II, MSA-3)
Cytoplasmic fluorescence patterns
e1
Fine speckled cytoplasmic
e2
Diffuse cytoplasmic
e3
Mitochondrial-like
e4
Lysosomal-like
e5
Golgi-like
e6
Actin-like
e7
Vimentin-like
=
Nucleoplasmic patterns
b1
Homogeneous/positive nucleoli
b2
Homogeneous/negative nucleoli
b3
Large speckled (nuclear matrix)
b4
Coarse speckled
b5
Fine speckled
b6
Grainy Scl-70-like
b7
Pleomorphic speckled (PCNA)
b8
Centromere
b9
Multiple nuclear dots (NSp-I)
b10
Coiled body
d1
d2
d3
d4
d5
g
5
1
/
,
9
9
0
8
/
0
,
7
6
4
1
5
4
3
1
0
/
.
-
,
M
=
a
a2
Smooth membranous
Punctate membranous
Anti-GBM disease
Antiphospholipid Syndrome, primary
Antiphospholipid Syndrome, secondary
Anti-Synthetase Syndrome
Churg-Strauss Syndrome
Dermatomyositis
Drug induced lupus
Granulomatosis with polyangiitis (former Wegener granulomatosis)
Inflammatory bowel disease
Microscopic polyangiitis
Mixed connective tissue disease
Polymyositis
Primary biliary cirrhosis
!
!
!
!
!
!
!
!
!
!
Rheumatoid arthritis
Scleroderma, diffuse
Scleroderma, limited
Scleroderma /Myositis overlap
Similar to limited scleroderma
Sjögren Syndrome, primary
Sjögren Syndrome, secondary
Subacute cutanous lupus erythematosus
Systemic lupus erythematosus
Vasculitis, systemic
J
I
J
L
J
I
G
E